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KMID : 0613820060160060925
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Journal of Life Science
2006 Volume.16 No. 6 p.925 ~ p.931
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Effects of ENA-A(ENA actimineral resource A) Ion Water on the Activity and Differentiation of MC3T3-E1 Osteoblastic cell
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Lee Ji-Won
Jeon Sang-Kyung
Kim Hyun-Jeong
Lee In-Seon
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Abstract
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Culture of osteoblast is extremely valuable in analyzing biological features that are specific to bone. ENA-A, ENA actimineral resource A, is a seaweed origin alkaline water. To investigate the bioactivity of ENA which act on bone metabolism, we studied the effects of a ENA on the activity of osteoblast MC3T3-E1 cells. ENA (1, 2, 4%) dose-dependently increased survival (p＜0.05) and alkaline phosphatase activity (p＜0.05) on MC3T3-E1 cell. And examined histochemistry and nodule formation according to the time course. To determine the expression patterns of bone-related proteins during the MC3T3-E1 osteoblast-like cell differentiation by using RT-PCR. This study suggest that ENA may promote the function of osteoblastic cells and play an important role in bone formation.
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KEYWORD
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ENA, osteoblast, bone formation, proliferation, alkaline phosphatase, mineralization
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